annexin v binding buffer (Miltenyi Biotec)
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annexin v binding buffer
Annexin V Binding Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Annexin+V+Binding+Buffer/pm42288492-222-10-13
Average 97 stars, based on 89 article reviews
Annexin V Binding Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/Annexin+V+Binding+Buffer/pm42288492-222-10-13
Average 97 stars, based on 89 article reviews
annexin v binding buffer - by Bioz Stars,
2026-09
97/100 stars
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Binding Assay:Article Title: Human brain cell types shape host-rabies virus transcriptional interactions revealing a preexisting pro-viral astrocyte subpopulation Article Snippet: Samples were added to the freshly washed LS columns (130-042-401, Miltenyi Biotec). .. Columns were washed with 4.5 mL Article Title: Transmembrane thiol isomerase TMX1 inhibits fibrin formation by down-regulation of platelet phosphatidylserine exposure Article Snippet: .. The cell pellet was suspended with 90 μl Article Title: Precision oncology in a dish: patient-derived glioma organoids to guide novel therapeutic strategies Article Snippet: Ten GOs per condition were collected after 48 h treatment, washed with 1X PBS, dissociated in collagenase IV (20 min, 37 °C), filtered through a 70 μm strainer, and resuspended in 100 μL Annexin V Binding Buffer (BioLegend, USA). .. Samples were stained with 5 μL Annexin V–FITC (BioLegend, USA) and 5 μL PI (Sigma-Aldrich, USA) for 15 min at RT, diluted with 400 μL Article Title: Human brain cell types shape host-rabies virus transcriptional interactions revealing a preexisting pro-viral astrocyte subpopulation. Article Snippet: Samples were added to the freshly washed LS columns (130-042-401, Miltenyi Biotec). .. Columns were washed with 4.5 mL Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death Article Snippet: The cancer cells were incubated for 4 h with or without recombinant human IL-2 IS of research grade (2 μg/mL) (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, the cells were washed with the Article Title: Precision oncology in a dish: patient-derived glioma organoids to guide novel therapeutic strategies. Article Snippet: Annexin V / Propidium Iodide (PI) apoptosis assay Ten GOs per condition were collected after 48 h treatment, washed with PBS, dissociated in collagenase IV (20 min, 37 °C), filtered through a 70 μm strainer, and resuspended in 100 μL Annexin V Binding Buffer (BioLegend, USA). .. Samples were stained with 5 μL Annexin V–FITC (BioLegend, USA) and 5 μL PI (Sigma-Aldrich, USA) for 15 min at room temperature, diluted with 400 μL Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA-RNA sequencing. Article Snippet: Cells were resuspended in 100 μl of bead solution from a Dead Cell Removal kit (Miltenyi Biotec, 130-090- 101) and incubated for 15 min in the dark. .. Article Title: Transmembrane thiol isomerase TMX1 inhibits fibrin formation by down-regulation of platelet phosphatidylserine exposure. Article Snippet: .. The cell pellet was suspended with 90 μl Staining:Article Title: Precision oncology in a dish: patient-derived glioma organoids to guide novel therapeutic strategies Article Snippet: Ten GOs per condition were collected after 48 h treatment, washed with 1X PBS, dissociated in collagenase IV (20 min, 37 °C), filtered through a 70 μm strainer, and resuspended in 100 μL Annexin V Binding Buffer (BioLegend, USA). .. Samples were stained with 5 μL Annexin V–FITC (BioLegend, USA) and 5 μL PI (Sigma-Aldrich, USA) for 15 min at RT, diluted with 400 μL Article Title: Precision oncology in a dish: patient-derived glioma organoids to guide novel therapeutic strategies. Article Snippet: Annexin V / Propidium Iodide (PI) apoptosis assay Ten GOs per condition were collected after 48 h treatment, washed with PBS, dissociated in collagenase IV (20 min, 37 °C), filtered through a 70 μm strainer, and resuspended in 100 μL Annexin V Binding Buffer (BioLegend, USA). .. Samples were stained with 5 μL Annexin V–FITC (BioLegend, USA) and 5 μL PI (Sigma-Aldrich, USA) for 15 min at room temperature, diluted with 400 μL Flow Cytometry:Article Title: Precision oncology in a dish: patient-derived glioma organoids to guide novel therapeutic strategies Article Snippet: Ten GOs per condition were collected after 48 h treatment, washed with 1X PBS, dissociated in collagenase IV (20 min, 37 °C), filtered through a 70 μm strainer, and resuspended in 100 μL Annexin V Binding Buffer (BioLegend, USA). .. Samples were stained with 5 μL Annexin V–FITC (BioLegend, USA) and 5 μL PI (Sigma-Aldrich, USA) for 15 min at RT, diluted with 400 μL Article Title: Precision oncology in a dish: patient-derived glioma organoids to guide novel therapeutic strategies. Article Snippet: Annexin V / Propidium Iodide (PI) apoptosis assay Ten GOs per condition were collected after 48 h treatment, washed with PBS, dissociated in collagenase IV (20 min, 37 °C), filtered through a 70 μm strainer, and resuspended in 100 μL Annexin V Binding Buffer (BioLegend, USA). .. Samples were stained with 5 μL Annexin V–FITC (BioLegend, USA) and 5 μL PI (Sigma-Aldrich, USA) for 15 min at room temperature, diluted with 400 μL Incubation:Article Title: IL-2 Receptor Expression in Renal Cell Carcinoma Cells: IL-2 Influences Cell Survival and Induces Cell Death Article Snippet: The cancer cells were incubated for 4 h with or without recombinant human IL-2 IS of research grade (2 μg/mL) (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Subsequently, the cells were washed with the |
![PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1053/pmc13011053/pmc13011053__gr3.jpg)